Disulfide crosslinks to probe the structure and flexibility of a designed four-helix bundle protein.
نویسندگان
چکیده
The introduction of disulfide crosslinks is a generally useful method by which to identify regions of a protein that are close together in space. Here we describe the use of disulfide crosslinks to investigate the structure and flexibility of a family of designed 4-helix bundle proteins. The results of these analyses lend support to our working model of the proteins' structure and suggest that the proteins have limited main-chain flexibility.
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عنوان ژورنال:
- Protein science : a publication of the Protein Society
دوره 3 12 شماره
صفحات -
تاریخ انتشار 1994